UNIT 7.15 Pyrosequencing: Powerful and Quantitative Sequencing Technology
Published Online: 11 OCT 2013
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Kreutz, M., Hochstein, N., Kaiser, J., Narz, F. and Peist, R. 2013. Pyrosequencing: Powerful and Quantitative Sequencing Technology. Current Protocols in Molecular Biology. 104:7.15.1–7.15.23.
- Published Online: 11 OCT 2013
Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification. In this unit, we describe a complete workflow from sample to result that is suitable for each of these applications. As cytosine conversion is a key element of successful methylation analysis using pyrosequencing, a support protocol for bisulfite treatment is also included. Curr. Protoc. Mol. Biol. 104:7.15.1-7.15.23. © 2013 by John Wiley & Sons, Inc.
- DNA methylation;
- genome analysis;