Unit

UNIT 7.15 Pyrosequencing: Powerful and Quantitative Sequencing Technology

  1. Martin Kreutz,
  2. Norbert Hochstein,
  3. Julia Kaiser,
  4. Frank Narz,
  5. Ralf Peist

Published Online: 11 OCT 2013

DOI: 10.1002/0471142727.mb0715s104

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Kreutz, M., Hochstein, N., Kaiser, J., Narz, F. and Peist, R. 2013. Pyrosequencing: Powerful and Quantitative Sequencing Technology. Current Protocols in Molecular Biology. 104:7.15:7.15.1–7.15.23.

Author Information

  1. Qiagen GmbH, Hilden, Germany

Publication History

  1. Published Online: 11 OCT 2013

Abstract

Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification. In this unit, we describe a complete workflow from sample to result that is suitable for each of these applications. As cytosine conversion is a key element of successful methylation analysis using pyrosequencing, a support protocol for bisulfite treatment is also included. Curr. Protoc. Mol. Biol. 104:7.15.1-7.15.23. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • pyrosequencing;
  • DNA methylation;
  • SNP;
  • AQ;
  • genome analysis;
  • sequencing