UNIT 7.17 Next-Generation Sequencing Fragment Library Construction
Published Online: 1 JUL 2014
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Podnar, J., Deiderick, H. and Hunicke-Smith, S. 2014. Next-Generation Sequencing Fragment Library Construction. Current Protocols in Molecular Biology. 107:7.17:7.17.1–7.17.16.
- Published Online: 1 JUL 2014
All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5′ and 3′ flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results. Curr. Protoc. Mol. Biol. 107:7.17.1-7.17.16. © 2014 by John Wiley & Sons, Inc.