UNIT 7.17 Next-Generation Sequencing Fragment Library Construction

  1. Jessica Podnar,
  2. Heather Deiderick,
  3. Scott Hunicke-Smith

Published Online: 1 JUL 2014

DOI: 10.1002/0471142727.mb0717s107

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Podnar, J., Deiderick, H. and Hunicke-Smith, S. 2014. Next-Generation Sequencing Fragment Library Construction. Current Protocols in Molecular Biology. 107:7.17:7.17.1–7.17.16.

Author Information

  1. Genomic Sequencing and Analysis Facility, University of Texas at Austin, Austin, Texas

Publication History

  1. Published Online: 1 JUL 2014


All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5′ and 3′ flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results. Curr. Protoc. Mol. Biol. 107:7.17.1-7.17.16. © 2014 by John Wiley & Sons, Inc.


  • NGS;
  • library;
  • libraries;
  • sequencing;
  • DNA;
  • fragment;
  • shotgun;
  • amplicon