UNIT 9.3 Transfection by Electroporation

  1. Huntington Potter

Published Online: 1 MAY 2003

DOI: 10.1002/0471142727.mb0903s62

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Potter, H. 2003. Transfection by Electroporation. Current Protocols in Molecular Biology. 62:I:9.3:9.3.1–9.3.6.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: APR 2003

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Electroporation--the use of high-voltage electric shocks to introduce DNA into cells--can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques. In this unit, the Basic Protocol describes the electroporation of mammalian cells and an Alternate Protocol outlines modifications for preparation and transfection of plant protoplasts.