UNIT 9.3 Transfection by Electroporation
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Potter, H. 2003. Transfection by Electroporation. Current Protocols in Molecular Biology. 62:I:9.3:9.3.1–9.3.6.
- Published Online: 1 MAY 2003
- Published Print: APR 2003
This is not the most recent version of the article. View current version (1 OCT 2010)
Electroporation--the use of high-voltage electric shocks to introduce DNA into cells--can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques. In this unit, the Basic Protocol describes the electroporation of mammalian cells and an Alternate Protocol outlines modifications for preparation and transfection of plant protoplasts.