UNIT 9.3 Transfection by Electroporation
Published Online: 1 OCT 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Potter, H. and Heller, R. 2010. Transfection by Electroporation. Current Protocols in Molecular Biology. 92:9.3.1–9.3.10.
- Published Online: 1 OCT 2010
- Published Print: OCT 2010
Electroporation—the use of high-voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques. This unit describes electroporation of mammalian cells, including ES cells for the preparation of knock-out, knock-in, and transgenic mice. Protocols are described for the use of electroporation in vivo to perform gene therapy for cancer therapy and DNA vaccination. Also described are modifications for preparation and transfection of plant protoplasts. Curr. Protoc. Mol. Biol. 92:9.3.1-9.3.10. © 2010 by John Wiley & Sons, Inc.
- molecular biology;
- introduction of DNA into cells;
- gene regulation;
- gene expression;
- transcription and translation;
- gene therapy;
- DNA vaccine