Unit

UNIT 9.4 Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents

  1. Pamela Hawley-Nelson,
  2. Valentina Ciccarone

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0904s48

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hawley-Nelson, P. and Ciccarone, V. 2001. Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents. Current Protocols in Molecular Biology. 9:I:9.4.

Author Information

  1. Life Technologies, Inc., Rockville, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999

This is not the most recent version of the article. View current version (1 JAN 2008)

Abstract

The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage. Transfection mediated by cationic lipids is commonly used in industrial protein production as well as in some clinical gene therapy protocols. Replacing our previous unit on this topic, this new version describes how to perform transfection of adherent and suspension cells, insect cells, and RNA transfection using the cationic lipid system.