UNIT 9.4 Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Hawley-Nelson, P. and Ciccarone, V. 2001. Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents. Current Protocols in Molecular Biology. 9:I:9.4.
- Published Online: 1 MAY 2001
- Published Print: OCT 1999
This is not the most recent version of the article. View current version (1 JAN 2008)
The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage. Transfection mediated by cationic lipids is commonly used in industrial protein production as well as in some clinical gene therapy protocols. Replacing our previous unit on this topic, this new version describes how to perform transfection of adherent and suspension cells, insect cells, and RNA transfection using the cationic lipid system.