Unit

UNIT 9.4 Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents

  1. Pamela Hawley-Nelson1,
  2. Valentina Ciccarone2,
  3. Meredith L. Moore3

Published Online: 1 JAN 2008

DOI: 10.1002/0471142727.mb0904s81

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hawley-Nelson, P., Ciccarone, V. and Moore, M. L. 2008. Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents. Current Protocols in Molecular Biology. 81:I:9.4:9.4.1–9.4.17.

Author Information

  1. 1

    Centocor, Inc., Radnor, Pennsylvania

  2. 2

    MacroGenics, Inc., Rockville, Maryland

  3. 3

    Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 JAN 2008
  2. Published Print: JAN 2008

Abstract

The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage. Transfection mediated by cationic lipids is commonly used in industrial protein production as well as in some clinical gene therapy protocols. This unit describes how to perform transfection of adherent and suspension cells, insect cells, and RNA transfection using cationic lipid reagents. Curr. Protoc. Mol. Biol. 81:9.4.1-9.4.17. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • transfection;
  • luciferase;
  • cationic lipid;
  • cell culture;
  • transgene;
  • transient gene expression;
  • stable gene expression