UNIT 9.6 Overview of Genetic Reporter Systems

  1. Steven R. Kain1,
  2. Subinay Ganguly (table)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0906s36

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Kain, S. R. and Ganguly, S. 2001. Overview of Genetic Reporter Systems. Current Protocols in Molecular Biology. 68:II:9.6:9.6.1–9.6.12.

Author Information

  1. 1

    Clontech Laboratories, Inc., Palo Alto, California

  2. 2

    SmithKline Beecham, King of Prussia, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1996


One method to gauge changes in transcription is to link a presumed cis-acting sequence(s) from a gene of interest to the coding sequence for an unrelated reporter gene. Following introduction of the chimeric reporter construct into an appropriate cell type or animal, measurement of reporter-gene product provides an indirect estimate of the induction in gene expression directed by the regulatory sequences. Numerous in vitro and in vivo reporters genes are discussed in this overview with regard to important issues regarding the selection of a transcription reporter gene, and their applications and limitations.