UNIT 9.6 Overview of Genetic Reporter Systems
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Kain, S. R. and Ganguly, S. 2001. Overview of Genetic Reporter Systems. Current Protocols in Molecular Biology. 68:II:9.6:9.6.1–9.6.12.
- Published Online: 1 MAY 2001
- Published Print: OCT 1996
One method to gauge changes in transcription is to link a presumed cis-acting sequence(s) from a gene of interest to the coding sequence for an unrelated reporter gene. Following introduction of the chimeric reporter construct into an appropriate cell type or animal, measurement of reporter-gene product provides an indirect estimate of the induction in gene expression directed by the regulatory sequences. Numerous in vitro and in vivo reporters genes are discussed in this overview with regard to important issues regarding the selection of a transcription reporter gene, and their applications and limitations.