Unit

UNIT 9.7B Nonisotopic Assays for Reporter Gene Activity

  1. Allan R. Brasier1,
  2. John J. Fortin2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0907bs29

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Brasier, A. R. and Fortin, J. J. 2001. Nonisotopic Assays for Reporter Gene Activity. Current Protocols in Molecular Biology. 29:II:9.7B:9.7.12–9.7.21.

Author Information

  1. 1

    University of Texas, Galveston, Texas

  2. 2

    Tropix, Inc., Bedford, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1995

Abstract

This unit describes two nonisotopic systems for reporter gene activity in cells transfected with the firefly luciferase expression plasmid or the β-galactosidase expression plasmid. Both of these chemiluminescent assays have the advantages of high sensitivity and broad linear range. In the chemiluminescent detection procedures given in this unit, both luciferase activity and β-galactosidase activity can be measured with either a luminometer or a scintillation counter.