UNIT 9.7B Nonisotopic Assays for Reporter Gene Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Brasier, A. R. and Fortin, J. J. 2001. Nonisotopic Assays for Reporter Gene Activity. Current Protocols in Molecular Biology. 29:II:9.7B:9.7.12–9.7.21.
- Published Online: 1 MAY 2001
- Published Print: JAN 1995
This unit describes two nonisotopic systems for reporter gene activity in cells transfected with the firefly luciferase expression plasmid or the β-galactosidase expression plasmid. Both of these chemiluminescent assays have the advantages of high sensitivity and broad linear range. In the chemiluminescent detection procedures given in this unit, both luciferase activity and β-galactosidase activity can be measured with either a luminometer or a scintillation counter.