Unit

UNIT 9.9 Overview of the Retrovirus Transduction System

  1. Constance Cepko1,
  2. Warren Pear2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0909s36

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Cepko, C. and Pear, W. 2001. Overview of the Retrovirus Transduction System. Current Protocols in Molecular Biology. 36:III:9.9:9.9.1–9.9.16.

Author Information

  1. 1

    Harvard Medical School, Boston, Massachusetts

  2. 2

    University of Pennsylvania, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1996

Abstract

A retrovirus vector is an infectious virus used to introduce a nonviral gene into mitotic cells in vivo or in vitro. The efficient and precise integration machinery of naturally occurring retroviruses is utilized to produce either a single copy or a few copies of the viral genome stably integrated into a host chromosome. This unit presents an overview of the retrovirus life cycle and a description of vector designs and packaging cell lines.