UNIT 9.9 Overview of the Retrovirus Transduction System
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Cepko, C. and Pear, W. 2001. Overview of the Retrovirus Transduction System. Current Protocols in Molecular Biology. 36:III:9.9:9.9.1–9.9.16.
- Published Online: 1 MAY 2001
- Published Print: OCT 1996
A retrovirus vector is an infectious virus used to introduce a nonviral gene into mitotic cells in vivo or in vitro. The efficient and precise integration machinery of naturally occurring retroviruses is utilized to produce either a single copy or a few copies of the viral genome stably integrated into a host chromosome. This unit presents an overview of the retrovirus life cycle and a description of vector designs and packaging cell lines.