UNIT 9.13 Detection of Helper Virus in Retrovirus Stocks
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Cepko, C. and Pear, W. 2001. Detection of Helper Virus in Retrovirus Stocks. Current Protocols in Molecular Biology. 36:III:9.13:9.13.1–9.13.7.
- Published Online: 1 MAY 2001
- Published Print: OCT 1996
Helper virus is a replication-competent virus that is sometimes present in stocks of replication-incompetent virus. There are several types of applications in which the presence of helper virus can be problematic. If animal infections are being done, helper virus can lead to leukemia, particularly if the infection is carried out pre- or neonatally. If retroviruses are being used for lineage analysis, helper virus may cause horizontal spread of the marker virus, creating false lineage relationships. This unit describes protocols for the detection of helper virus by a selectable marker assay, by rescue of an integrated provirus, or by measuring reverse transcriptase activity.