Unit

UNIT 9.14 Retrovirus Infection of Cells In Vitro and In Vivo

  1. Constance Cepko1,
  2. Warren Pear2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0914s36

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Cepko, C. and Pear, W. 2001. Retrovirus Infection of Cells In Vitro and In Vivo. Current Protocols in Molecular Biology. 36:III:9.14:9.14.1–9.14.6.

Author Information

  1. 1

    Harvard Medical School, Boston, Massachusetts

  2. 2

    University of Pennsylvania, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1996

Abstract

There are many applications in which retrovirus vectors are used as transduction agents. In some cases, the vector carries a gene that one wishes to express in a target cell in order to study the function of that gene. In other cases, the virus is used to introduce a histochemical marker gene into cells in order to follow their fate. Retrovirus vectors can also be used in a variety of cells type to investigate regulatory sequences in which a reporter gene and regulatory sequences are carried by the vector and to immortalize or transform primary cells by transduction of oncogenes. For each application, the infection protocol may vary and must often be optimized. Guidelines for infection of cells in some typical in vivo and in vitro experiments are presented in this overview.