UNIT 9.15 Human Somatic Cell Gene Targeting
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Waldman, T., Lee, C., Nishanian, T. G. and Kim, J.-S. 2003. Human Somatic Cell Gene Targeting. Current Protocols in Molecular Biology. 62:IV:9.15:9.15.1–9.15.20.
- Published Online: 1 MAY 2003
- Published Print: APR 2003
Human somatic cell gene targeting provides a powerful tool to scientists studying gene function in cultured human cells. This technology allows scientists to knock out genes in human somatic cells in a fashion analogous to the creation of knockout mice. Human somatic cell gene targeting brings the power of genetics to the study of human genes in human cells by making it possible to compare cells or individuals that are genetically identical except for a single, well-defined mutation in an endogenous gene. These modified cells can be studied both in vitro and in vivo. This unit presents protocols for human somatic cell gene targeting.