Unit

UNIT 9.15 Human Somatic Cell Gene Targeting

  1. Todd Waldman,
  2. Carolyn Lee,
  3. Tagvor G. Nishanian,
  4. Jung-Sik Kim

Published Online: 1 MAY 2003

DOI: 10.1002/0471142727.mb0915s62

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Waldman, T., Lee, C., Nishanian, T. G. and Kim, J.-S. 2003. Human Somatic Cell Gene Targeting. Current Protocols in Molecular Biology. 62:IV:9.15:9.15.1–9.15.20.

Author Information

  1. Georgetown University School of Medicine, Washington, D.C.

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: APR 2003

Abstract

Human somatic cell gene targeting provides a powerful tool to scientists studying gene function in cultured human cells. This technology allows scientists to knock out genes in human somatic cells in a fashion analogous to the creation of knockout mice. Human somatic cell gene targeting brings the power of genetics to the study of human genes in human cells by making it possible to compare cells or individuals that are genetically identical except for a single, well-defined mutation in an endogenous gene. These modified cells can be studied both in vitro and in vivo. This unit presents protocols for human somatic cell gene targeting.