UNIT 10.3 Two-Dimensional Gel Electrophoresis Using the ISO-DALT System
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Adams, L. D. 2001. Two-Dimensional Gel Electrophoresis Using the ISO-DALT System. Current Protocols in Molecular Biology. 20:II:10.3:10.3.1–10.3.12.
- Published Online: 1 MAY 2001
- Published Print: OCT 1992
Two high-resolution electrophoretic procedures (isoelectric focusing and SDS-polyacrylamide gel electrophoresis) are combined in this unit to provide much greater resolution than either of the individual procedures. Solubilized proteins are first separated according to their isoelectric point by isoelectric focusing in a tube gel. This first dimension gel is then applied to the top of an SDS-polyacrylamide slab gel and electrophoresed. The proteins in the first-dimension gel migrate into the second-dimension gel where they are further separated on the basis of their molecular size. The ISO-DALT system was specifically designed for running multiple high-resolution two-dimensional gels at one time.