Unit

UNIT 10.6 Staining Proteins in Gels

  1. Joachim Sasse1,
  2. Sean R. Gallagher2

Published Online: 1 AUG 2003

DOI: 10.1002/0471142727.mb1006s63

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Sasse, J. and Gallagher, S. R. 2003. Staining Proteins in Gels. Current Protocols in Molecular Biology. 63:III:10.6:10.6.1–10.6.25.

Author Information

  1. 1

    Shriners Hospital for Crippled Children, Tampa, Florida

  2. 2

    UVP, Inc., Upland, California

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUL 2003

This is not the most recent version of the article. View current version (1 JAN 2009)

Abstract

This unit describes protocols for detecting protein in a gel by either Coomassie blue staining or silver staining. The former is easier and more rapid; however, silver staining methods are considerably more sensitive and thus can be used to detect smaller amounts of protein. Alternate rapid staining procedures are provided for each method and a support protocol describes how to photograph stained gels. Fluorescent staining is a popular alternative to traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Staining of proteins in SDS-polyacrylamide gels is described, and an alternate protocol details variations in the procedure for proteins in nondenaturing gels. A final support protocol describes the photography of fluorescently stained proteins.