Unit

UNIT 10.6 Staining Proteins in Gels

  1. Joachim Sasse1,
  2. Sean R. Gallagher2

Published Online: 1 JAN 2009

DOI: 10.1002/0471142727.mb1006s85

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Sasse, J. and Gallagher, S. R. 2009. Staining Proteins in Gels. Current Protocols in Molecular Biology. 85:III:10.6:10.6.1–10.6.27.

Author Information

  1. 1

    Shriners Hospital for Crippled Children, Tampa, Florida

  2. 2

    UVP, Inc., Upland, California

Publication History

  1. Published Online: 1 JAN 2009
  2. Published Print: JAN 2009

Abstract

This unit describes protocols for detecting protein in a gel by Coomassie blue, silver, or fluorescent staining. As a general protein stain, Coomassie is easier and more rapid; however, fluorescent and silver staining methods are considerably more sensitive and thus can be used to detect smaller amounts of protein. Fluorescent staining is a popular alternative to traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Alternate protocols describe rapid Coomassie and silver staining methods, as well as fluorescent stains that are specific for phosphoproteins and glycoproteins. Staining of proteins in SDS-polyacrylamide gels is described; variations for fluorescent staining of proteins in nondenaturing gels are also included. Support protocols describe photography of stained proteins. Curr. Protoc. Mol. Biol. 85:10.6.1-10.6.27. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • silver;
  • Coomassie;
  • SYPRO;
  • electrophoresis;
  • protein;
  • staining