UNIT 10.6 Staining Proteins in Gels
Published Online: 1 JAN 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Sasse, J. and Gallagher, S. R. 2009. Staining Proteins in Gels. Current Protocols in Molecular Biology. 85:III:10.6:10.6.1–10.6.27.
- Published Online: 1 JAN 2009
- Published Print: JAN 2009
This unit describes protocols for detecting protein in a gel by Coomassie blue, silver, or fluorescent staining. As a general protein stain, Coomassie is easier and more rapid; however, fluorescent and silver staining methods are considerably more sensitive and thus can be used to detect smaller amounts of protein. Fluorescent staining is a popular alternative to traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Alternate protocols describe rapid Coomassie and silver staining methods, as well as fluorescent stains that are specific for phosphoproteins and glycoproteins. Staining of proteins in SDS-polyacrylamide gels is described; variations for fluorescent staining of proteins in nondenaturing gels are also included. Support protocols describe photography of stained proteins. Curr. Protoc. Mol. Biol. 85:10.6.1-10.6.27. © 2009 by John Wiley & Sons, Inc.