Unit

UNIT 10.7 Detection of Proteins on Blot Transfer Membranes

  1. Joachim Sasse1,
  2. Sean R. Gallagher2

Published Online: 1 OCT 2008

DOI: 10.1002/0471142727.mb1007s84

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Sasse, J. and Gallagher, S. R. 2008. Detection of Proteins on Blot Transfer Membranes. Current Protocols in Molecular Biology. 84:III:10.7:10.7.1–10.7.6.

Author Information

  1. 1

    Shriners Hospital for Crippled Children, Tampa, Florida

  2. 2

    UVP, Inc., Upland, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008

Abstract

The staining of proteins bound to a transfer membrane can be useful for determining the efficiency of transfer and marking the location of molecular weight standards. This unit describes three methods for staining blots, using India ink, gold labeling, and fluorescent labeling with SYPRO Ruby. Detection limits of each staining method are given along with a list of compatible blot transfer membranes and gels. A support protocol describes a method for enhancement of staining using alkali treatment. Curr. Protoc. Mol. Biol. 84:10.7.1-10.7.6. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • nylon;
  • nitrocellulose;
  • PVDF;
  • protein/blotting;
  • stain;
  • total protein