UNIT 10.7 Detection of Proteins on Blot Transfer Membranes
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Sasse, J. and Gallagher, S. R. 2008. Detection of Proteins on Blot Transfer Membranes. Current Protocols in Molecular Biology. 84:III:10.7:10.7.1–10.7.6.
- Published Online: 1 OCT 2008
- Published Print: OCT 2008
The staining of proteins bound to a transfer membrane can be useful for determining the efficiency of transfer and marking the location of molecular weight standards. This unit describes three methods for staining blots, using India ink, gold labeling, and fluorescent labeling with SYPRO Ruby. Detection limits of each staining method are given along with a list of compatible blot transfer membranes and gels. A support protocol describes a method for enhancement of staining using alkali treatment. Curr. Protoc. Mol. Biol. 84:10.7.1-10.7.6. © 2008 by John Wiley & Sons, Inc.
- total protein