UNIT 10.9 Gel-Filtration Chromatography

  1. Lars Hagel

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1009s44

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hagel, L. 2001. Gel-Filtration Chromatography. Current Protocols in Molecular Biology. 44:IV:10.9:10.9.1–10.9.2.

Author Information

  1. Amersham Pharmacia Biotech AB, Uppsala, Sweden

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


Gel filtration (GF) chromatography separates proteins solely on the basis of molecular size. Separation is achieved using a porous matrix to which the molecules, for steric reasons, have different degrees of access--i.e., smaller molecules have greater access and larger molecules are excluded from the matrix. Hence, proteins are eluted from the GF column in decreasing order of size. This unit describes the experimental theory behind gel filtration and contains many useful tables listing the properties and characteristics of currently available matrices.