UNIT 10.10 Ion-Exchange Chromatography

  1. Alan Williams,
  2. Verna Frasca

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1010s44

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Williams, A. and Frasca, V. 2001. Ion-Exchange Chromatography. Current Protocols in Molecular Biology. 44:IV:10.10:10.10.1–10.10.30.

Author Information

  1. Amersham Pharmacia Biotech, Piscatraway, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


Ion-exchange chromatography separates biomolecules on the basis of charge characteristics. Charged groups on the surface of a protein interact with oppositely charged groups immobilized on the ion-exchange medium. This unit provides thorough explanations of the theory behind the technology, troubleshooting tips for making the procedure work best for you, and detailed listings of column matrices and separation properties.