UNIT 10.10 Ion-Exchange Chromatography
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Williams, A. and Frasca, V. 2001. Ion-Exchange Chromatography. Current Protocols in Molecular Biology. 44:IV:10.10:10.10.1–10.10.30.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
Ion-exchange chromatography separates biomolecules on the basis of charge characteristics. Charged groups on the surface of a protein interact with oppositely charged groups immobilized on the ion-exchange medium. This unit provides thorough explanations of the theory behind the technology, troubleshooting tips for making the procedure work best for you, and detailed listings of column matrices and separation properties.