UNIT 10.11A Immunoaffinity Chromatography
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Springer, T. A. 2001. Immunoaffinity Chromatography. Current Protocols in Molecular Biology. 36:IV:10.11A:10.11.1–10.11.9.
- Published Online: 1 MAY 2001
- Published Print: OCT 1996
Immunoaffinity purification is a powerful technique for isolating proteins. Purifications of 10,000-fold or more can often be achieved in one step. This unit describes the isolation of soluble or membrane-bound protein antigens from cells or homogenized tissue by immunoaffinity chromatography. The technique involves the elution of a single protein from an immunoaffinity column after prior elution of nonspecifically adsorbed proteins. An alternate protocol describes the use of batch purification of antigens which can shorten the column loading time.