UNIT 10.12 HPLC of Peptides and Proteins: Preparation and System Set-Up
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Boysen, R. I. and Hearn, M. T. 2001. HPLC of Peptides and Proteins: Preparation and System Set-Up. Current Protocols in Molecular Biology. 54:V:10.12:10.12.1–10.12.9.
- Published Online: 1 MAY 2001
- Published Print: APR 2001
The eight basic modes of HPLC currently in use for peptide and protein analysis and purification are presented in this overview. They include: size-exclusion chromatography (HP-SEC), ion-exchange chromatography (HP-IEX), normal phase chromatography (HP-NPC), hydrophobic interaction chromatography (HP-HIC), reversed-phase chromatography (RP-HPLC), hydrophilic interaction chromatography (HP-HILIC), immobilized metal ion affinity chromatography (HP-IMAC), and biospecific/biomimetic affinity chromatography (HP-BAC). In addition, some subsets of these chromatographic modes, e.g., mixed mode chromatography (HP-MMC), charge transfer chromatography (HP-CTC), or ligand-exchange chromatography (HP-LEC) are described. The unit includes overviews of the system requirements and planning strategies for set-up of the systems.