UNIT 10.18 Metabolic Labeling with Amino Acids

  1. Juan S. Bonifacino

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1018s44

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Bonifacino, J. S. 2001. Metabolic Labeling with Amino Acids. Current Protocols in Molecular Biology. 44:VI:10.18:10.18.1–10.18.10.

Author Information

  1. National Institute of Child Health and Human Development, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This update focuses on pulse-labeling and pulse-chase experiments done with [35S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds.