Unit

UNIT 10.19 Isolation of Proteins for Microsequence Analysis

  1. Malcolm Moss Jr.

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1019s21

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Moss, M. 2001. Isolation of Proteins for Microsequence Analysis. Current Protocols in Molecular Biology. 21:VI:10.19:10.19.1–10.19.12.

Author Information

  1. Center for Biologics Evaluation & Research, Food and Drug Administration, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1993

Abstract

This unit presents a method for determining the amino-terminal sequence of polypeptides or proteins. It is particularly appropriate for large fragments of insoluble or hydrophobic proteins or proteins that cannot be purified to >90% molar purity without electrophoresis. If the protein is blocked at the amino terminus, chemical cleavage or partial enzymatic digestion must be performed prior to electrophoresis. Upon isolation, the internal amino acid sequence is analyzed as described.