UNIT 10.19 Isolation of Proteins for Microsequence Analysis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Moss, M. 2001. Isolation of Proteins for Microsequence Analysis. Current Protocols in Molecular Biology. 21:VI:10.19:10.19.1–10.19.12.
- Published Online: 1 MAY 2001
- Published Print: JAN 1993
This unit presents a method for determining the amino-terminal sequence of polypeptides or proteins. It is particularly appropriate for large fragments of insoluble or hydrophobic proteins or proteins that cannot be purified to >90% molar purity without electrophoresis. If the protein is blocked at the amino terminus, chemical cleavage or partial enzymatic digestion must be performed prior to electrophoresis. Upon isolation, the internal amino acid sequence is analyzed as described.