Unit

UNIT 10.22 Protein Identification and Characterization by Mass Spectrometry

  1. Scott D. Patterson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1022s41

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Patterson, S. D. 2001. Protein Identification and Characterization by Mass Spectrometry. Current Protocols in Molecular Biology. 41:VI:10.22:10.22.1–10.22.24.

Author Information

  1. Amgen, Inc., Thousand Oaks, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1998

Abstract

This overview describes some of the new technologies that can be employed to facilitate rapid identification and characterization of proteins, including the use of correlative approaches for protein identification, rapid posttranslational modification analysis, identification of components in complex mixtures, and direct mass analysis of gel-separated proteins. The mass spectrometric methods referred to in this overview include matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and electrospray ionization mass spectrometry (ESI-MS).