UNIT 10.22 Protein Identification and Characterization by Mass Spectrometry
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Patterson, S. D. 2001. Protein Identification and Characterization by Mass Spectrometry. Current Protocols in Molecular Biology. 41:VI:10.22:10.22.1–10.22.24.
- Published Online: 1 MAY 2001
- Published Print: JAN 1998
This overview describes some of the new technologies that can be employed to facilitate rapid identification and characterization of proteins, including the use of correlative approaches for protein identification, rapid posttranslational modification analysis, identification of components in complex mixtures, and direct mass analysis of gel-separated proteins. The mass spectrometric methods referred to in this overview include matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and electrospray ionization mass spectrometry (ESI-MS).