Unit

UNIT 10.26 Isolation of Proteins by Heat-Induced Extraction from Formalin-Fixed, Paraffin-Embedded Tissue and Preparation of Tryptic Peptides for Mass Spectrometric Analysis

  1. Yuan Tian,
  2. Hui Zhang

Published Online: 1 APR 2010

DOI: 10.1002/0471142727.mb1026s90

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Tian, Y. and Zhang, H. 2010. Isolation of Proteins by Heat-Induced Extraction from Formalin-Fixed, Paraffin-Embedded Tissue and Preparation of Tryptic Peptides for Mass Spectrometric Analysis. Current Protocols in Molecular Biology. 90:VI:10.26:10.26.1–10.26.7.

Author Information

  1. Johns Hopkins University, Baltimore, Maryland

Publication History

  1. Published Online: 1 APR 2010
  2. Published Print: APR 2010

Abstract

This unit describes a method of isolating of proteins from formalin-fixed and paraffin-embedded (FFPE) tissue for mass spectrometry analysis. Heat-induced antigen retrieval is the basis of the protein extraction strategy presented in this protocol. This protocol may be used to identify nuclear, cytosolic, and membrane proteins from FFPE tissues extracted from tissue blocks or slides. Curr. Protoc. Mol. Biol. 90:10.26.1-10.26.7. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • peptide extraction;
  • formalin-fixed and paraffin-embedded tissue;
  • mass spectrometry;
  • protein identification