Unit

UNIT 10.30 Assays for Investigating deSUMOylation Enzymes

  1. Ikenna G. Madu,
  2. Yuan Chen

Published Online: 1 JUL 2012

DOI: 10.1002/0471142727.mb1030s99

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Madu, I. G. and Chen, Y. 2012. Assays for Investigating deSUMOylation Enzymes. Current Protocols in Molecular Biology. 99:VI:10.30:10.30.1–10.30.13.

Author Information

  1. Department of Molecular Medicine, Beckman Research Institute of the City of Hope, Duarte, California

Publication History

  1. Published Online: 1 JUL 2012
  2. Published Print: JUL 2012

Abstract

Post-translational modifications by the SUMO (Small Ubiquitin-like MOdifier) family of proteins are recently discovered essential regulatory mechanisms. All SUMO proteins are synthesized as larger precursors that are matured by SUMO-specific proteases, known as SENPs, which remove several C-terminal amino acids of SUMO to expose the Gly-Gly motif. SENPs also remove SUMO modifications from target proteins, making this modification highly dynamic. At least six deSUMOylation enzymes, all of which are encoded by essential genes, have been identified in mammals. SENP1 has been shown to play an important role in the development of prostate cancer and in angiogenesis. This unit describes and discusses methods for characterizing the deSUMOylation enzymes. These assays enable the identification of inhibitors of these enzymes and investigation of their mechanism of inhibition in order to develop research tools and future therapeutics. Curr. Protoc. Mol. Biol. 99:10.30.1-10.30.13. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • SUMO;
  • SENP;
  • vinyl sulfone;
  • FRET;
  • bioluminescence