UNIT 11.1 Conjugation of Enzymes to Antibodies
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Winston, S. E., Fuller, S. A., Evelegh, M. J. and Hurrell, J. G. 2001. Conjugation of Enzymes to Antibodies. Current Protocols in Molecular Biology. 50:I:11.1:11.1.1–11.1.7.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site of the antibody nor the active site of the enzyme is functionally altered. This unit describes procedures for cross-linking HRPO, urease or alkaline phosphatase to immunoaffinity-purified monoclonal or polyclonal antibodies (IgG).