UNIT 11.1 Conjugation of Enzymes to Antibodies

  1. Scott E. Winston1,
  2. Steven A. Fuller1,
  3. Michael J. Evelegh1,
  4. John G.R. Hurrell2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1101s50

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Winston, S. E., Fuller, S. A., Evelegh, M. J. and Hurrell, J. G. 2001. Conjugation of Enzymes to Antibodies. Current Protocols in Molecular Biology. 50:I:11.1:11.1.1–11.1.7.

Author Information

  1. 1

    ADI Diagnostics, Rexdale, Ontario

  2. 2

    Boehringer Mannheim, Indianapolis, Indiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000


Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site of the antibody nor the active site of the enzyme is functionally altered. This unit describes procedures for cross-linking HRPO, urease or alkaline phosphatase to immunoaffinity-purified monoclonal or polyclonal antibodies (IgG).