UNIT 11.3 Isotype Determination of Antibodies

  1. Peter Hornbeck (sandwich ELISA)1,
  2. Thomas A. Fleisher2,
  3. Nicholas M. Papadopoulos (electrophoresis and immunofixation)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1103s18

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hornbeck, P., Fleisher, T. A. and Papadopoulos, N. M. 2001. Isotype Determination of Antibodies. Current Protocols in Molecular Biology. 18:I:11.3:11.3.1–11.3.6.

Author Information

  1. 1

    University of Maryland, Baltimore, Maryland

  2. 2

    Warren Grant Magnuson Clinical Center, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1992


Frequently it is necessary to know the amount and serological class of antibodies made by an immunized animal, produced by hybridomas, or present in the serum of patients with inflammatory or neoplastic conditions. The immunologist's approach to such a problem is to consider the antibody or immunoglobulin molecules themselves as antigens and to use anti-immunoglobulin antibodies as the specific and sensitive agents of detection. This unit describes two methods for measurement and classification of supernatant or serum immunoglobulins: an ELISA and a method employing electrophoresis and immunofixation.