Unit

UNIT 11.4 Immunization of Mice

  1. Steven A. Fuller1,
  2. Miyoko Takahashi1,
  3. John G.R. Hurrell2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1104s18

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Immunization of Mice. Current Protocols in Molecular Biology. 18:II:11.4:11.4.1–11.4.6.

Author Information

  1. 1

    ADI Diagnostics, Rexdale, Ontario

  2. 2

    Boehringer Mannheim Diagnostics, Indianapolis, Indiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1992

Abstract

In the protocols in this unit, antigen is prepared for injection either by emulsifying an antigen solution with Freunds adjuvant or by homogenizing a polyacrylamide gel slice containing the protein antigen. Mice are immunized at 2- to 3-week intervals. Test bleeds are collected 7 days after each booster immunization to monitor serum antibody levels. Mice are chosen for hybridoma fusions when a sufficient antibody titer is reached.