Unit

UNIT 11.5 Preparation of Myeloma Cells

  1. Steven A. Fuller,
  2. Miyoko Takahashi,
  3. John G.R. Hurrell

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1105s18

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Preparation of Myeloma Cells. Current Protocols in Molecular Biology. 18:II:11.5:11.5.1–11.5.3.

Author Information

  1. Allelix Inc., Mississauga, Ontario

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1992

Abstract

In this unit, myeloma cells are cultured to ensure their sensitivity to the HAT selection medium used after cell fusion. Cell culture conditions are adjusted such that the Sp2/0 cells are in the log phase of growth and exhibit high viability at the time of collection for fusion. A support protocol is provided to determine the number of viable cells present in the cell culture.