UNIT 11.5 Preparation of Myeloma Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Preparation of Myeloma Cells. Current Protocols in Molecular Biology. 18:II:11.5:11.5.1–11.5.3.
- Published Online: 1 MAY 2001
- Published Print: APR 1992
In this unit, myeloma cells are cultured to ensure their sensitivity to the HAT selection medium used after cell fusion. Cell culture conditions are adjusted such that the Sp2/0 cells are in the log phase of growth and exhibit high viability at the time of collection for fusion. A support protocol is provided to determine the number of viable cells present in the cell culture.