Unit

UNIT 11.6 Preparation of Mouse Feeder Cells for Fusion and Cloning

  1. Steven A. Fuller,
  2. Miyoko Takahashi,
  3. John G.R. Hurrell

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1106s01

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Preparation of Mouse Feeder Cells for Fusion and Cloning. Current Protocols in Molecular Biology. 1:II:11.6:11.6.1–11.6.3.

Author Information

  1. Allelix Inc., Mississauga, Ontario

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1988

Abstract

To maximize the yield of hybrids from fusion and cloning procedures, feeder cells are required to be cocultured with the hybrids, while hybrid cell density is low. Mouse peritoneal cells, most of which are macrophages, have been found to be convenient and effective feeder cells which are a source of soluble growth factors for hybridoma cells.