UNIT 11.6 Preparation of Mouse Feeder Cells for Fusion and Cloning
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Preparation of Mouse Feeder Cells for Fusion and Cloning. Current Protocols in Molecular Biology. 1:II:11.6:11.6.1–11.6.3.
- Published Online: 1 MAY 2001
- Published Print: JAN 1988
To maximize the yield of hybrids from fusion and cloning procedures, feeder cells are required to be cocultured with the hybrids, while hybrid cell density is low. Mouse peritoneal cells, most of which are macrophages, have been found to be convenient and effective feeder cells which are a source of soluble growth factors for hybridoma cells.