UNIT 11.7 Fusion of Myeloma Cells with Immune Spleen Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Fusion of Myeloma Cells with Immune Spleen Cells. Current Protocols in Molecular Biology. 1:II:11.7:11.7.1–11.7.4.
- Published Online: 1 MAY 2001
- Published Print: JAN 1988
Liquid nitrogen storage is the method of choice for long-term safekeeping of hybridoma cell lines. Frozen aliquots of originally isolated hybridomas provide insurance against loss of antibody production and vigor during culture. There are many variations of cell freezing methods in use. However, for freezing and recovering hybridomas and lymphoid cells in general, the protocol described in this unit is simple and has been successful.