Unit

UNIT 11.8 Cloning of Hybridoma Cell Lines by Limiting Dilution

  1. Steven A. Fuller,
  2. Miyoko Takahashi,
  3. John G.R. Hurrell

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1108s01

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Cloning of Hybridoma Cell Lines by Limiting Dilution. Current Protocols in Molecular Biology. 1:II:11.8:11.8.1–11.8.2.

Author Information

  1. Allelix Inc., Mississauga, Ontario

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1988

Abstract

Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.