UNIT 11.8 Cloning of Hybridoma Cell Lines by Limiting Dilution
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Cloning of Hybridoma Cell Lines by Limiting Dilution. Current Protocols in Molecular Biology. 1:II:11.8:11.8.1–11.8.2.
- Published Online: 1 MAY 2001
- Published Print: JAN 1988
Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.