Unit

UNIT 11.9 Freezing and Recovery of Hybridoma Cell Lines

  1. Steven A. Fuller,
  2. Miyoko Takahashi,
  3. John G.R. Hurrell

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1109s01

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Freezing and Recovery of Hybridoma Cell Lines. Current Protocols in Molecular Biology. 1:II:11.9:11.9.1–11.9.3.

Author Information

  1. Allelix Inc., Mississauga, Ontario, Canada

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1988

Abstract

Liquid nitrogen storage is the method of choice for long-term safekeeping of hybridoma cell lines. Frozen aliquots of originally isolated hybridomas provide insurance against loss of antibody production and vigor during culture. There are many variations of cell freezing methods in use. However, for freezing and recovering hybridomas and lymphoid cells in general, the protocol described in this unit is simple and has been successful.