UNIT 11.10 Production of Monoclonal Antibody Supernatant and Ascites Fluid
Published Online: 1 JUL 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Yokoyama, W. M. 2008. Production of Monoclonal Antibody Supernatant and Ascites Fluid. Current Protocols in Molecular Biology. 83:II:11.10:11.10.1–11.10.10.
- Published Online: 1 JUL 2008
- Published Print: JUL 2008
This unit details methods for production of monoclonal antibodies. Two methods are given for production of hybridoma supernatants, including one for large-scale production. A protocol for large-scale production of hybridomas or cell lines is presented for use in isolation of cellular proteins. Finally, a method is given for producing and obtaining mouse ascites fluid containing monoclonal antibody. Recommendations developed by the Committee on Methods of Producing Monoclonal Antibodies (Institute of Laboratory Animal Research, National Research Council) on the use of animals for producing monoclonal antibodies are also discussed. These recommendations are designed to foster the judicious use of animals and to strongly encourage the use of in vitro methods whenever possible. Curr. Protoc. Mol. Biol. 83:11.10.1-11.10.10. © 2008 by John Wiley & Sons, Inc.
- antibody production;