Unit

UNIT 11.11 Purification of Monoclonal Antibodies

  1. Steven A. Fuller,
  2. Miyoko Takahashi,
  3. John G.R. Hurrell

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1111s37

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Purification of Monoclonal Antibodies. Current Protocols in Molecular Biology. 37:II:11.11:11.11.1–11.11.5.

Author Information

  1. Allelix Inc., Mississauga, Ontario

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1997

Abstract

The uses of monoclonal antibodies as enzyme conjugates and as immunoaffinity reagents require their purification from the crude ascites fluid. This unit provides protocols for purification using protein A-Sepharose chromatography and affinity chromatography, which are superior to ammonium sulfate precipitation, gel filtration, or ion-exchange chromatography for the preparation of contaminant-free antibody.