UNIT 11.11 Purification of Monoclonal Antibodies
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Fuller, S. A., Takahashi, M. and Hurrell, J. G. 2001. Purification of Monoclonal Antibodies. Current Protocols in Molecular Biology. 37:II:11.11:11.11.1–11.11.5.
- Published Online: 1 MAY 2001
- Published Print: JAN 1997
The uses of monoclonal antibodies as enzyme conjugates and as immunoaffinity reagents require their purification from the crude ascites fluid. This unit provides protocols for purification using protein A-Sepharose chromatography and affinity chromatography, which are superior to ammonium sulfate precipitation, gel filtration, or ion-exchange chromatography for the preparation of contaminant-free antibody.