Unit

UNIT 11.14 Purification of Immunoglobulin G Fraction from Antiserum, Ascites Fluid, or Hybridoma Supernatant

  1. Helen M. Cooper1,
  2. Yvonne Paterson2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1114s50

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Cooper, H. M. and Paterson, Y. 2001. Purification of Immunoglobulin G Fraction from Antiserum, Ascites Fluid, or Hybridoma Supernatant. Current Protocols in Molecular Biology. 50:III:11.14:11.14.1–11.14.5.

Author Information

  1. 1

    Walter and Eliza Hall Institute, Melbourne, Australia

  2. 2

    University of Pennsylvania, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000

Abstract

This unit describes the isolation of the immunoglobulin G (IgG) fraction (containing antibodies of all specificities) from a complex protein mixture such as antiserum, ascites fluid, or hybridoma supernatant. The basic procedure utilizes saturated ammonium sulfate solution to precipitate the IgG fraction, while an alternate protocol describes fractionation of IgG by chromatography on DEAE-Affi-Gel Blue resin.