UNIT 12.13 Engineering Designer Nucleases with Customized Cleavage Specificities
Published Online: 1 OCT 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Sander, J. D., Maeder, M. L. and Joung, J. K. 2011. Engineering Designer Nucleases with Customized Cleavage Specificities. Current Protocols in Molecular Biology. 96:12.13:12.13.1–12.13.16.
- Published Online: 1 OCT 2011
- Published Print: OCT 2011
Engineered designer nucleases can be used to efficiently modify genomic sequence in a wide variety of model organisms and cell types. Zinc finger nucleases (ZFNs), consisting of an engineered zinc finger array fused to a non-specific cleavage domain, have been extensively used to modify a broad range of endogenous genes. Protocols for engineering ZFNs targeted to specific gene sequences of interest using the context-dependent assembly (CoDA) method are described in this unit. Curr. Protoc. Mol. Biol. 96:12.13.1-12.13.16. © 2011 by John Wiley & Sons, Inc.
- zinc finger nucleases;
- zinc finger proteins;
- context-dependent assembly;
- protein engineering;
- DNA-binding domains