UNIT 13.2B Drug-Induced Permeabilization of S. cerevisiae

  1. Maria D. Mayan

Published Online: 1 OCT 2010

DOI: 10.1002/0471142727.mb1302bs92

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Mayan, M. D. 2010. Drug-Induced Permeabilization of S. cerevisiae. Current Protocols in Molecular Biology. 92:I:13.2B:13.2B.1–13.2B.4.

Author Information

  1. Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College, London, United Kingdom

Publication History

  1. Published Online: 1 OCT 2010
  2. Published Print: OCT 2010


Budding yeast are not permeable to many drugs. This unit provides a protocol in which polygodial is used to permeabilize the cell membrane, thereby allowing budding yeast cells to be treated with drugs that otherwise would be ineffective. Curr. Protoc. Mol. Biol. 92:13.2B.1-13.2B.4. © 2010 by John Wiley & Sons, Inc.


  • permeabilization;
  • Alpha-amantin;
  • DRB;
  • S. cerevisiae