UNIT 13.2 Growth and Manipulation of Yeast

  1. Douglas A. Treco1,
  2. Fred Winston2

Published Online: 1 APR 2008

DOI: 10.1002/0471142727.mb1302s82

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Treco, D. A. and Winston, F. 2008. Growth and Manipulation of Yeast. Current Protocols in Molecular Biology. 82:I:13.2:13.2.1–13.2.12.

Author Information

  1. 1

    Massachusetts General Hospital, Boston, Massachusetts

  2. 2

    Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 APR 2008
  2. Published Print: APR 2008


Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures. This unit describes culture conditions for these basic techniques. Additional methods describe determination of yeast mating type, diploid construction, sporulation, tetrad dissection, and random spore analysis. Curr. Protoc. Mol. Biol. 82:13.2.1-13.2.12. © 2008 by John Wiley & Sons, Inc.


  • yeast;
  • media;
  • yeast culture;
  • growth