UNIT 13.3B EMS and UV Mutagenesis in Yeast

  1. Fred Winston

Published Online: 1 APR 2008

DOI: 10.1002/0471142727.mb1303bs82

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Winston, F. 2008. EMS and UV Mutagenesis in Yeast. Current Protocols in Molecular Biology. 82:I:13.3B:13.3B.1–13.3B.5.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 APR 2008
  2. Published Print: APR 2008


Many fundamental biological processes have been elucidated by the isolation and analysis of mutants that are defective in such processes. Therefore, the methods to generate mutants are of great importance in model organisms. This unit describes two protocols for mutagenesis of yeast—using ethyl methanesulfate (EMS) and ultraviolet (UV) light. Each of these methods has been used successfully for many years. Curr. Protoc. Mol. Biol. 82:13.3B.1-13.3B.5. © 2008 by John Wiley & Sons, Inc.


  • yeast;
  • mutagenesis;
  • EMS;
  • UV