UNIT 13.3B EMS and UV Mutagenesis in Yeast
Published Online: 1 APR 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Winston, F. 2008. EMS and UV Mutagenesis in Yeast. Current Protocols in Molecular Biology. 82:I:13.3B:13.3B.1–13.3B.5.
- Published Online: 1 APR 2008
- Published Print: APR 2008
Many fundamental biological processes have been elucidated by the isolation and analysis of mutants that are defective in such processes. Therefore, the methods to generate mutants are of great importance in model organisms. This unit describes two protocols for mutagenesis of yeast—using ethyl methanesulfate (EMS) and ultraviolet (UV) light. Each of these methods has been used successfully for many years. Curr. Protoc. Mol. Biol. 82:13.3B.1-13.3B.5. © 2008 by John Wiley & Sons, Inc.