Unit

UNIT 13.6 Yeast Vectors and Assays for Expression of Cloned Genes

  1. Ann Reynolds1,
  2. Victoria Lundblad2,
  3. David Dorris3,
  4. Marie Keaveney (filter lift assay)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1306s39

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Reynolds, A., Lundblad, V., Dorris, D. and Keaveney, M. 2001. Yeast Vectors and Assays for Expression of Cloned Genes. Current Protocols in Molecular Biology. 39:II:13.6:13.6.1–13.6.6.

Author Information

  1. 1

    University of Washington, Seattle, Washington

  2. 2

    Baylor College of Medicine, Houston, Texas

  3. 3

    Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1997

Abstract

This unit describes some of the most commonly used yeast vectors, as well as the cloned yeast genes that form the basis for these plasmids. Yeast vectors can be grouped into five general classes, based on their mode of replication in yeast: YIp, YRp, YCp, YEp, and YLp plasmids. With the exception of the YLp plasmids (yeast linear plasmids), all of these plasmids can be maintained in E. coli as well as in S. cerevisiae and thus are referred to as shuttle vectors. The nomenclature of different classes of yeast vectors, as well as details about their mode of replication in yeast are discussed.