UNIT 13.6 Yeast Vectors and Assays for Expression of Cloned Genes
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Reynolds, A., Lundblad, V., Dorris, D. and Keaveney, M. 2001. Yeast Vectors and Assays for Expression of Cloned Genes. Current Protocols in Molecular Biology. 39:II:13.6:13.6.1–13.6.6.
- Published Online: 1 MAY 2001
- Published Print: JUL 1997
This unit describes some of the most commonly used yeast vectors, as well as the cloned yeast genes that form the basis for these plasmids. Yeast vectors can be grouped into five general classes, based on their mode of replication in yeast: YIp, YRp, YCp, YEp, and YLp plasmids. With the exception of the YLp plasmids (yeast linear plasmids), all of these plasmids can be maintained in E. coli as well as in S. cerevisiae and thus are referred to as shuttle vectors. The nomenclature of different classes of yeast vectors, as well as details about their mode of replication in yeast are discussed.