Unit

UNIT 13.7 Introduction of DNA into Yeast Cells

  1. Daniel M. Becker1,
  2. Victoria Lundblad2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1307s27

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Becker, D. M. and Lundblad, V. 2001. Introduction of DNA into Yeast Cells. Current Protocols in Molecular Biology. 27:III:13.7:13.7.1–13.7.10.

Author Information

  1. 1

    Stanford Law School, Stanford, California

  2. 2

    Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1994

Abstract

The most commonly used yeast transformation protocol is the lithium acetate procedure (described here). It is reasonably fast and provides a transformation efficiency of 105 to 106 transformants/μg. This efficiency rivals that achieved for most, but not all, strains with the more difficult and time-consuming spheroplast procedure presented here. However, the fastest and easiest of the transformation methods is electroporation, as described in this unit. For a number of strains, electroporation offers the highest transformation efficiency, and may prove especially useful with limiting quantities of transforming DNA. Unlike the lithium acetate procedure, however, electroporation saturates at low DNA levels, restricting its general utility.