UNIT 13.7 Introduction of DNA into Yeast Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Becker, D. M. and Lundblad, V. 2001. Introduction of DNA into Yeast Cells. Current Protocols in Molecular Biology. 27:III:13.7:13.7.1–13.7.10.
- Published Online: 1 MAY 2001
- Published Print: JUL 1994
The most commonly used yeast transformation protocol is the lithium acetate procedure (described here). It is reasonably fast and provides a transformation efficiency of 105 to 106 transformants/μg. This efficiency rivals that achieved for most, but not all, strains with the more difficult and time-consuming spheroplast procedure presented here. However, the fastest and easiest of the transformation methods is electroporation, as described in this unit. For a number of strains, electroporation offers the highest transformation efficiency, and may prove especially useful with limiting quantities of transforming DNA. Unlike the lithium acetate procedure, however, electroporation saturates at low DNA levels, restricting its general utility.