UNIT 13.10B Conditional Depletion of Nuclear Proteins by the Anchor Away System
Published Online: 1 JAN 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Fan, X., Geisberg, J. V., Wong, K. H. and Jin, Y. 2011. Conditional Depletion of Nuclear Proteins by the Anchor Away System. Current Protocols in Molecular Biology. 93:III:13.10B:13.10B.1–13.10B.8.
- Published Online: 1 JAN 2011
- Published Print: JAN 2011
Nuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus. Curr. Protoc. Mol. Biol. 93:13.10B.1-13.10B.8. © 2011 by John Wiley & Sons, Inc.
- Anchor Away;
- conditional depletion of nuclear proteins