Unit

UNIT 13.10B Conditional Depletion of Nuclear Proteins by the Anchor Away System

  1. Xiaochun Fan,
  2. Joseph V. Geisberg,
  3. Koon Ho Wong,
  4. Yi Jin

Published Online: 1 JAN 2011

DOI: 10.1002/0471142727.mb1310bs93

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fan, X., Geisberg, J. V., Wong, K. H. and Jin, Y. 2011. Conditional Depletion of Nuclear Proteins by the Anchor Away System. Current Protocols in Molecular Biology. 93:III:13.10B:13.10B.1–13.10B.8.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 JAN 2011
  2. Published Print: JAN 2011

Abstract

Nuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus. Curr. Protoc. Mol. Biol. 93:13.10B.1-13.10B.8. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • Anchor Away;
  • conditional depletion of nuclear proteins