Unit

UNIT 13.11 Preparation of Yeast DNA

  1. Charles S. Hoffman

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1311s39

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hoffman, C. S. 2001. Preparation of Yeast DNA. Current Protocols in Molecular Biology. 39:13.11.1–13.11.4.

Author Information

  1. Boston College, Chestnut Hill, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1997

Abstract

Molecular studies in yeast often require the isolation of both plasmid and chromosomal yeast DNA. Plasmid DNA is used in the transformation of E. coli, whereas chromosomal DNA is used for Southern hybridization analysis, in vitro amplification by the polymerase chain reaction (PCR), or cloning of integrated plasmids. This unit presents two variations of the “smash and grab” protocol that produce suitable DNA for all these applications. These protocols work for both Saccharomyces cerevisiae and Schizosaccharomyces pombe.